安捷伦的全外芯片捕获的目标区域的bed文件是可以下载的,下载网址

https://earray.chem.agilent.com/suredesign

这个网站我没保存过,毕竟用的频率不高,但每次想用的时候还要搜一下这个网站(Σ( ° △ °|||)︴)

注册登录之后,找到Find Design -> SureSelect DNA -> Agilent Catalog Designs,进一步筛选之后,可以下载对应的文件。

BED files

You can import these files into a compatible genome browser to graphically view the locations of the tracks in the genome.

[design ID]_Covered.bed - This BED file contains a single track of the genomic regions that are covered by one or more probes in the design. The fourth column of the file contains annotation information. You can use this file for assessing coverage metrics.

[design ID]_Padded.bed - This BED file contains a single track of the genomic regions that you can expect to sequence when using the design for target enrichment. To determine these regions, the program extends the regions in the Covered BED file by 100 bp on each side.

[design ID]_Regions.bed - This BED file contains a single track of the target regions of the design. For catalog designs, the track in this file is identical to the track in the Covered BED file.

NOTE Some SureSelect catalog designs have not been annotated to gene databases. For these designs, the gene annotation information is missing from the Covered BED file.

Text files

You can view the text files in any text editor program (e.g. NotePad) or spreadsheet program (e.g. Excel).

[design ID]_Probes.txt - This file is a list of the probes in the design, with specific information about each probe, including its probe ID, sequence, genomic coordinates, and the target it is intended to capture.

[design ID]_Targets.txt - This file contains a list of the database identifiers to which the probes in this design were annotated.

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