Generate Accurate Consensus Sequences from a Single SMRTbell
文章目录
bax2bam
bax2bam -o mynewbam mydata.1.bax.h5 mydata.2.bax.h5 mydata.3.bax.h5
circus consensus sequence
ccs –minLength=100 myData.subreads.bam myResult.bam
进行CCS由pacbio测序系统决定的,插入序列测多遍之后,可以用来校正随机错误。
参考
https://github.com/PacificBiosciences/unanimity/blob/develop/doc/PBCCS.md
文章作者 zzx
上次更新 2019-03-22