Generate Accurate Consensus Sequences from a Single SMRTbell

Circular-Consensus-Sequence

bax2bam

bax2bam -o mynewbam mydata.1.bax.h5 mydata.2.bax.h5 mydata.3.bax.h5

circus consensus sequence

Circular-Consensus-Sequence

ccs –minLength=100 myData.subreads.bam myResult.bam

进行CCS由pacbio测序系统决定的,插入序列测多遍之后,可以用来校正随机错误。

参考

https://github.com/PacificBiosciences/unanimity/blob/develop/doc/PBCCS.md

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